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2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. Ciência Rural
Alves,Ivanilda dos Santos; Carmazini,Valéria Cristina Barbosa; Santos,Cosme Damião dos; Almeida,Julieta Andrea Silva de.
ABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cultivar Mundo Novo; 2-iP; Somatic embryos; Embryogenic structures.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100403
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Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique Acta Botanica
Padilha,João Henrique Delfrate; Ribas,Luciana Lopes Fortes; Amano,Érika; Quoirin,Marguerite.
Considering the necessity of Acrocomia aculeata propagation for large-scale production, the aim of this study was to establish a somatic embryogenesis protocol using the thin cell layer (TCL) technique. Aerial parts of in vitro plants were transversally cut at the base into eight TCLs and placed in a culture medium for callus induction. The induction medium was composed of Y3 salts and Morel´s vitamins and supplemented with 150, 300 or 600 μM picloram. After 12 weeks the calli were transferred to a medium supplemented with BAP or 2-iP (12.5 or 25 μM). After 18 weeks, the somatic embryo clusters were transferred to a conversion medium (plant growth regulator-free medium). Primary callus induction rate was higher in the first three TCLs and in media...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-iP; Callus induction; Histology; Macaw palm; Picloram.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400516
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